771 Novel approach for skin regeneration: mRNA-based telomerase enhancement of autologous skin cell suspension

The RECELL® Autologous Cell Harvesting Device enables point-of-care preparation of autologous skin cell suspension (ASCS). Currently, ASCS is approved for the treatment acute thermal burn injuries, with additional indications under investigation. We hypothesize therapeutic potential may be enhanced regeneration and primed rejuvenation by improving cells’ ability to divide differentiate reverse-aging through telomere extension. A method safely extend telomeres in fibroblasts myoblasts has been demonstrated using delivery modified mRNA encoding telomerase (TERT). This increased activity, delayed expression senescence markers, proliferative capacity, while avoiding immortalization insertional mutagenesis. Notably, our previous studies showed TERT therapy reversed vascular extended lifespan progeria mice. purpose this work demonstrate feasibility modifying activity lipid nanoparticles (LNPs). uptake downstream protein were evaluated vitro various LNPs. Data from Incucyte FACS experiments onset mRNA-LNP cationic LNPs 15-30min, yielding transfection efficiency up 95% within 12h. Telomerase was identified transfected a TRAP assay, significant increase observed over control. Animal an immunocompromised mouse transplanted human derived are ongoing understand impact on regeneration. Methods have developed efficiently transfect substantially activity. novel approach holds promise other RNA-based cellular therapies aimed at cutaneous defects.